Identification_Information:
  Citation:
    Citation_Information:
      Originator: Christina A. Kellogg
      Publication_Date: 20151105
      Title: Microbial and environmental dataset from Crocker Reef, Florida Keys, 2014-2015
      Geospatial_Data_Presentation_Form: Tabular Digital Data
      Online_Linkage: http://coastal.er.usgs.gov/data-release/doi-F74Q7S25/data/Microbial_and_environmental_dataset_from_Crocker_Reef_Florida_Keys_2014_2015.txt
      Larger_Work_Citation:
        Citation_Information:
          Originator: Kellogg, Christina A.
          Originator: Yates, Kimberly K.
          Originator: Lawler, Stephanie N.
          Originator: Moore, Christopher S.
          Originator: Smiley, Nathan A.
          Publication_Date: 2015
          Title: Seasonal microbial and environmental parameters at Crocker Reef, Florida Keys, 2014-2015
          Geospatial_Data_Presentation_Form: Publication (Other)
          Series_Information:
            Series_Name: U.S. Geological Survey Open-File Report
            Issue_Identification: 2015-1203
          Publication_Information:
            Publication_Place: St. Petersburg, FL
            Publisher: U. S. Geological Survey
          Online_Linkage: https://pubs.er.usgs.gov/publication/ofr20151203
  Description:
    Abstract: Crocker Reef was the site of an integrated reefscape characterization effort focused on calcification and related biogeochemical processes as part of the USGS Coral Reef Ecosystem Study (CREST) project. This effort included two intensive seasonal sampling trips to capture summer (July 8 to 17, 2014) and winter (January 29 to February 5, 2015) conditions. This data release represents water column microbial and environmental data collected for use as metadata in future publications examining reef metabolic processes via metagenomes derived from water samples and fine-scale temporal and spatial carbonate chemistry measurements. Microbial data are total bacterial counts per milliliter seawater, total viral counts per milliliter of seawater, and plate counts using Thiosulfate Citrate Bile Salts Sucrose (TCBS)  agar of Vibrio (bacteria) per milliliter of seawater. Environmental data are water temperature, salinity, dissolved oxygen, and pH.
    Purpose: Data were collected for use as metadata in future publications examining reef metabolic processes via metagenomes derived from water samples and fine-scale temporal and spatial carbonate chemistry measurements. Data were acquired during USGS field activity number (FAN) 2014-315-FA and 2015-303-FA; additional survey details are available at http://cmgds.marine.usgs.gov/fan_info.php?fan=2014-315-FA and http://cmgds.marine.usgs.gov/fan_info.php?fan=2015-303-FA. This dataset, Crocker_datarelease_2014-2015.csv, is included in a USGS Data Release that can be accessed online at http://dx.doi.org/10.5066/F74Q7S25.
  Time_Period_of_Content:
    Time_Period_Information:
      Range_of_Dates/Times:
        Beginning_Date: 20140708
        Ending_Date: 20150205
    Currentness_Reference: observed
  Status:
    Progress: Complete
    Maintenance_and_Update_Frequency: not planned
  Spatial_Domain:
    Bounding_Coordinates:
      West_Bounding_Coordinate: -80.52775
      East_Bounding_Coordinate: -80.52644
      North_Bounding_Coordinate: 24.91083
      South_Bounding_Coordinate: 24.90897
  Keywords:
    Theme:
      Theme_Keyword_Thesaurus: USGS
      Theme_Keyword: water temperature
      Theme_Keyword: water quality
      Theme_Keyword: water pH
      Theme_Keyword: salinity
      Theme_Keyword: oxygen content (water)
      Theme_Keyword: bacteria
      Theme_Keyword: microbiology
      Theme_Keyword: viruses
    Theme:
      Theme_Keyword_Thesaurus: None
      Theme_Keyword: Vibrio
      Theme_Keyword: coral reef
    Place:
      Place_Keyword_Thesaurus: None
      Place_Keyword: Crocker Reef
      Place_Keyword: Islamorada
      Place_Keyword: Florida Keys
      Place_Keyword: Florida
  Access_Constraints: none
  Use_Constraints: The U.S. Geological Survey requests to be acknowledged as originators of the data in future products or derivative research.
  Point_of_Contact:
    Contact_Information:
      Contact_Person_Primary:
        Contact_Person: Christina Kellogg
        Contact_Organization: U.S. Geological Survey
      Contact_Address:
        Address_Type: Mailing and Physical
        Address: 600 4th Street S.
        City: St. Petersburg
        State_or_Province: Florida
        Postal_Code: 33701
        Country: USA
      Contact_Voice_Telephone: 727-502-8128
      Contact_Electronic_Mail_Address: ckellogg@usgs.gov
  Native_Data_Set_Environment: Environment as of Metadata Creation: Microsoft Windows 7 Version 6.1 (Build 7601) Service Pack 1; Esri ArcGIS 10.2.2 (Build 3552) Service Pack N/A (Build N/A)
Data_Quality_Information:
  Attribute_Accuracy:
    Attribute_Accuracy_Report: A YSI multimeter was used to measure temperature (+/-0.01 C), salinity (+/-0.01) and dissolved oxygen (+/-0.1 mg l-1). Samples were analyzed for total pH (pHT; +/-0.005) using an Ocean Optics spectrometer and thymol blue indicator dye. Bacterial and viral counts are averages of triplicate sample counts made on flow cytometry. Vibrio counts are averages of triplicate TCBS agar plate counts.
  Logical_Consistency_Report: No formal logical accuracy tests were conducted.
  Completeness_Report: Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details.
  Positional_Accuracy:
    Horizontal_Positional_Accuracy:
      Horizontal_Positional_Accuracy_Report: No formal positional accuracy tests were conducted.
    Vertical_Positional_Accuracy:
      Vertical_Positional_Accuracy_Report: A formal accuracy assessment of the vertical positional information in the dataset has either not been conducted, or is not applicable.
  Lineage:
    Process_Step:
      Process_Description: Data collected in the field were either analyzed in-situ or brought back to the St. Petersburg Coastal and Marine Science Center for subsequent lab analyses.
      Process_Date: 20150301
Spatial_Data_Organization_Information:
  Direct_Spatial_Reference_Method: Point
  Point_and_Vector_Object_Information:
    SDTS_Terms_Description:
      SDTS_Point_and_Vector_Object_Type: Entity point
      Point_and_Vector_Object_Count: 94
Spatial_Reference_Information:
  Horizontal_Coordinate_System_Definition:
    Geographic:
      Latitude_Resolution: 0.0197885540
      Longitude_Resolution: 0.0227288676
      Geographic_Coordinate_Units: Decimal degrees
    Geodetic_Model:
      Ellipsoid_Name: WGS 1984
      Semi-major_Axis: 6378137.0
      Denominator_of_Flattening_Ratio: 298.257223563
Entity_and_Attribute_Information:
  Detailed_Description:
    Entity_Type:
      Entity_Type_Label: Crocker_datarelease_2014-2015.csv
      Entity_Type_Definition: Microbial and environmental water column data from Crocker Reef, Florida, collected in 2014 and 2015.
      Entity_Type_Definition_Source: Producer defined
    Attribute:
      Attribute_Label: Sample_ID
      Attribute_Definition: Sample identification code, includes site, date, and time sample was collected.
      Attribute_Definition_Source: USGS
      Attribute_Domain_Values:
        Unrepresentable_Domain: Sample identification code, includes site, date, and time sample was collected.
    Attribute:
      Attribute_Label: Year
      Attribute_Definition: Year of data collection.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 2014
          Range_Domain_Maximum: 2015
    Attribute:
      Attribute_Label: Date
      Attribute_Definition: Date of sample collection.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 10-Jul
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 11-Jul
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 12-Jul
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 13-Jul
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 2-Feb
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 3-Feb
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 30-Jan
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 31-Jan
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 4-Feb
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 8-Jul
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 9-Jul
          Enumerated_Domain_Value_Definition: Calendar date using day, month format.
          Enumerated_Domain_Value_Definition_Source: Producer defined
    Attribute:
      Attribute_Label: Local_Time
      Attribute_Definition: Time of sample collection, in local time.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 11:30
          Enumerated_Domain_Value_Definition: Local time using 24-hour clock convention.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 15:30
          Enumerated_Domain_Value_Definition: Local time using 24-hour clock convention.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 19:30
          Enumerated_Domain_Value_Definition: Local time using 24-hour clock convention.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 23:30
          Enumerated_Domain_Value_Definition: Local time using 24-hour clock convention.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 3:30
          Enumerated_Domain_Value_Definition: Local time using 24-hour clock convention.
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 7:30
          Enumerated_Domain_Value_Definition: Local time using 24-hour clock convention.
          Enumerated_Domain_Value_Definition_Source: Producer defined
    Attribute:
      Attribute_Label: Time_Zone
      Attribute_Definition: Time zone used during data collection.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: EDT
          Enumerated_Domain_Value_Definition: Eastern Daylight Time (EDT)
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: EST
          Enumerated_Domain_Value_Definition: Eastern Standard Time (EST)
          Enumerated_Domain_Value_Definition_Source: Producer defined
    Attribute:
      Attribute_Label: Latitude
      Attribute_Definition: Latitude of the sample location in degrees (WGS84).
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 24.90897 N
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 24.90913 N
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 24.91082 N
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 24.91083 N
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
    Attribute:
      Attribute_Label: Longitude
      Attribute_Definition: Longitude of the sample location in decimal degrees (WGS84)
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 80.52644 W
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 80.52658 W
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 80.52758 W
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Enumerated_Domain:
          Enumerated_Domain_Value: 80.52775 W
          Enumerated_Domain_Value_Definition: World Geodetic System 1984 Geographic Coordinate System
          Enumerated_Domain_Value_Definition_Source: Producer defined
    Attribute:
      Attribute_Label: Approximate Depth (m)
      Attribute_Definition: Water depth at site, in meters. Approximate because the water is shallow and affected by tides.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 3.7
          Range_Domain_Maximum: 4.3
    Attribute:
      Attribute_Label: Virus/mL
      Attribute_Definition: Viral particles enumerated by flow cytometry per milliliter of seawater.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 1950000
          Range_Domain_Maximum: 23300000
    Attribute:
      Attribute_Label: Bacteria/mL
      Attribute_Definition: Bacterial particles enumerated by flow cytometry per milliliter of seawater.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 241000
          Range_Domain_Maximum: 826000
    Attribute:
      Attribute_Label: DO
      Attribute_Definition: Dissolved Oxygen
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 5.25
          Range_Domain_Maximum: 6.9
    Attribute:
      Attribute_Label: pH
      Attribute_Definition: Sample pH
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 0
          Range_Domain_Maximum: 8.12
    Attribute:
      Attribute_Label: Temperature (°C)
      Attribute_Definition: Sample temperature, in degrees Celsius.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 22.2
          Range_Domain_Maximum: 31.9
    Attribute:
      Attribute_Label: Salinity
      Attribute_Definition: Sample salinity
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Range_Domain:
          Range_Domain_Minimum: 36.30
          Range_Domain_Maximum: 37.57
    Attribute:
      Attribute_Label: Vibrio CFU/mL
      Attribute_Definition: Agar plate counts of Vibrio bacterial colony-forming units per milliliter of seawater.
      Attribute_Definition_Source: Producer defined
      Attribute_Domain_Values:
        Unrepresentable_Domain: Detectable count values ranged between 3.3 and 3060 CFU/mL. All other values were listed as: BLD - Below Limit of Detection, ND - Not determined or TNTC Too Numerous To Count.
  Overview_Description:
    Entity_and_Attribute_Overview: The entity and attribute information provided here describes the tabular data associated with the dataset. Please review the detailed descriptions that are provided (the individual attribute descriptions) for information on the values that appear as fields/table entries of the dataset.
    Entity_and_Attribute_Detail_Citation: The entity and attribute information was generated by the individual and/or agency identified as the originator of the dataset. Please review the rest of the metadata record for additional details and information.
Distribution_Information:
  Distributor:
    Contact_Information:
      Contact_Person_Primary:
        Contact_Person: Christina Kellogg
        Contact_Organization: U.S. Geological Survey
      Contact_Address:
        Address_Type: Mailing and Physical
        Address: 600 4th Street S.
        City: St. Petersburg
        State_or_Province: Florida
        Postal_Code: 33701
        Country: USA
      Contact_Voice_Telephone: 727-502-8128
      Contact_Electronic_Mail_Address: ckellogg@usgs.gov
  Distribution_Liability: Although these data have been processed successfully on a computer system at the USGS, no warranty expressed or implied is made regarding the display or utility of the data on any other system, or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS shall not be held liable for improper or incorrect use of the data described and/or contained herein. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
  Standard_Order_Process:
    Digital_Form:
      Digital_Transfer_Information:
        Format_Name: Tabular Digital Data
      Digital_Transfer_Option:
        Online_Option:
          Computer_Contact_Information:
            Network_Address:
              Network_Resource_Name: http://coastal.er.usgs.gov/data-release/doi-F74Q7S25/data/Crocker_datarelease_2014-2015.csv
    Fees: None. No fees are applicable for obtaining the dataset.
Metadata_Reference_Information:
  Metadata_Date: 20151104
  Metadata_Contact:
    Contact_Information:
      Contact_Person_Primary:
        Contact_Person: Christina Kellogg
        Contact_Organization: U.S. Geological Survey
      Contact_Address:
        Address_Type: Mailing and Physical
        Address: 600 4th Street S.
        City: St. Petersburg
        State_or_Province: Florida
        Postal_Code: 33701
        Country: USA
      Contact_Voice_Telephone: 727-502-8128
      Contact_Electronic_Mail_Address: ckellogg@usgs.gov
  Metadata_Standard_Name: FGDC Content Standard for Digital Geospatial Metadata
  Metadata_Standard_Version: FGDC-STD-001-1998
  Metadata_Use_Constraints: Public domain data from the U.S. Government are freely redistributable with proper metadata and source attribution. Please recognize the U.S. Geological Survey as the originator of the dataset.