project_name sample_name bioproject_id sample_title organism host collection_date geo_loc_name lat_lon samp_collect_device samp_mat_process source_material_id samp_size nucl_acid_ext nucl_acid_amp lib_size target_gene target_subfragment pcr_primers mid seq_meth depth temp samp_store_temp salinity "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.01Q7 PRJNA296835 ROV.2012.NF01.Q7 Coral metagenome Unclassified Anthothelidae 2012 """Atlantic Ocean; Baltimore Canyon""" 38.149448 N 73.837895 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 17266 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" CATAGTAGTG GS FLX 454 pyrosequencing 451 m 6.4 ûC neg 20 ûC 35.1 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.02Q7 PRJNA296835 ROV.2012.NF02.Q7 Coral metagenome Unclassified Anthothelidae 2012 """Atlantic Ocean; Baltimore Canyon""" 38.144950 N 73.834483 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 76 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" CGAGAGATAC GS FLX 454 pyrosequencing 401 m 6.8 ûC neg 20 ûC 35.1 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.13Q6 PRJNA296835 ROV.2012.NF13.Q6 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.161487 N 73.856465 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 29482 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" ACGAGTGCGT GS FLX 454 pyrosequencing 434 m 7.1 ûC neg 20 ûC 35.1 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.13Q7 PRJNA296835 ROV.2012.NF13.Q7 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.161070 N 73.856163 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 26072 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" ACGCTCGACA GS FLX 454 pyrosequencing 432 m 7.5 ûC neg 20 ûC 35.1 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.15Q6 PRJNA296835 ROV.2012.NF15.Q6 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.173512 N 73.841965 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 19011 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" AGACGCACTC GS FLX 454 pyrosequencing 416 m 7.2 ûC neg 20 ûC 35.1 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.15Q7 PRJNA296835 ROV.2012.NF15.Q7 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.175122 N 73.845125 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 24570 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" AGCACTGTAG GS FLX 454 pyrosequencing 457 m 6.8 ûC neg 20 ûC 35.1 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.16Q6 PRJNA296835 ROV.2012.NF16.Q6 Coral metagenome Unclassified Anthothelidae 2012 """Atlantic Ocean; Baltimore Canyon""" 38.181962 N 73.860835 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 12659 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" ATCAGACACG GS FLX 454 pyrosequencing 435 m 5.7 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.16Q7 PRJNA296835 ROV.2012.NF16.Q7 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.181985 N 73.861092 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 25074 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" ATATCGCGAG GS FLX 454 pyrosequencing 436 m 6.3 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.17Q6 PRJNA296835 ROV.2012.NF17.Q6 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.118832 N 73.847417 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 26826 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" CGTGTCTCTA GS FLX 454 pyrosequencing 575 m 5.7 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.17Q7 PRJNA296835 ROV.2012.NF17.Q7 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.118210 N 73.847688 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 10341 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" CTCGCGTGTC GS FLX 454 pyrosequencing 575 m 5.7 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.18Q6 PRJNA296835 ROV.2012.NF18.Q6 Coral metagenome Anthothela sp. 2012 """Atlantic Ocean; Baltimore Canyon""" 38.118158 N 73.849030 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 13048 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" TCTCTATGCG GS FLX 454 pyrosequencing 524 m 5.5 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" NF.18Q7 PRJNA296835 ROV.2012.NF18.Q7 Coral metagenome Anthothela grandiflora 2012 """Atlantic Ocean; Baltimore Canyon""" 38.117922 N 73.845465 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 11981 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" TGATACGTCT GS FLX 454 pyrosequencing 679 m 5.1 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.686Q2 PRJNA296835 ROV.2013.RB.686.Q2 Coral metagenome Unknown genus in Anthothelidae 2013 """Atlantic Ocean; Norfolk Canyon""" 37.058587 N 74.605852 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 978 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" ATACGACGTA GS FLX 454 pyrosequencing 480 m 6.6 ûC neg 20 ûC 35.1 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.686Q4 PRJNA296835 ROV.2013.RB.686.Q4 Coral metagenome Anthothela grandiflora 2013 """Atlantic Ocean; Norfolk Canyon""" 37.054690 N 74.603935 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 24844 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" TCACGTACTA GS FLX 454 pyrosequencing 581 m 5.9 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.686Q5 PRJNA296835 ROV.2013.RB.686.Q5 Coral metagenome Anthothela sp. 2013 """Atlantic Ocean; Norfolk Canyon""" 37.054699 N 74.603939 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 32484 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" CGTCTAGTAC GS FLX 454 pyrosequencing 581 m 5.9 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.687Q3 PRJNA296835 ROV.2013.RB.687.Q3 Coral metagenome Anthothela sp. 2013 """Atlantic Ocean; Norfolk Canyon""" 37.054881 N 74.577786 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 4646 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" TCTACGTAGC GS FLX 454 pyrosequencing 594 m 5.6 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.687Q4 PRJNA296835 ROV.2013.RB.687.Q4 Coral metagenome Unclassified Anthothelidae 2013 """Atlantic Ocean; Norfolk Canyon""" 37.053907 N 74.580567 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 10172 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" TGTACTACTC GS FLX 454 pyrosequencing 704 m 5.3 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.687Q5 PRJNA296835 ROV.2013.RB.687.Q5 Coral metagenome Anthothela grandiflora 2013 """Atlantic Ocean; Norfolk Canyon""" 37.054808 N 74.578777 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 14277 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" ACGACTACAG GS FLX 454 pyrosequencing 606 m 5.7 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.688Q1 PRJNA296835 ROV.2013.RB.688.Q1 Coral metagenome Anthothela grandiflora 2013 """Atlantic Ocean; Norfolk Canyon""" 37.024297 N 74.588163 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 14305 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" CGTAGACTAG GS FLX 454 pyrosequencing 559 m 5.8 ûC neg 20 ûC 34.9 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.688Q2 PRJNA296835 ROV.2013.RB.688.Q2 Coral metagenome Anthothela sp. 2013 """Atlantic Ocean; Norfolk Canyon""" 37.023429 N 74.592413 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 4345 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" TACGAGTATG GS FLX 454 pyrosequencing 474 m 6.5 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.688Q3 PRJNA296835 ROV.2013.RB.688.Q3 Coral metagenome Unknown genus in Anthothelidae 2013 """Atlantic Ocean; Norfolk Canyon""" 37.023538 N 74.592445 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 13827 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" ACGACTACAG GS FLX 454 pyrosequencing 474 m 6.4 ûC neg 20 ûC 35.0 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.688Q4 PRJNA296835 ROV.2013.RB.688.Q4 Coral metagenome Anthothela sp. 2013 """Atlantic Ocean; Norfolk Canyon""" 37.024201 N 74.588153 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 255315 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" CGTAGACTAG GS FLX 454 pyrosequencing 559 m 5.8 ûC neg 20 ûC 34.9 psu "16S amplicons from cold water coral microbiomes ofÊAnthothela grandiflora,ÊAnthothelaÊspp. and unknown genus" RB.688Q5 PRJNA296835 ROV.2013.RB.688.Q5 Coral metagenome Anthothela grandiflora 2013 """Atlantic Ocean; Norfolk Canyon""" 37.024247 N 74.588199 W Remotely-operated vehicle (ROV) Preserved using RNAlater overnight at 4 ûC before extended storage in -20 ûC Coral tissue ~ 50 mg doi:10.1371/journal.pone.0009554 doi:10.1093/nar/gkq873 298322 16S rRNA V4-V5 "FWD: 5_ AYTGGGYDTAAAGNG, REV:5_ CGTATCGCCTCCCTCGCGCCATCAG" TACGAGTATG GS FLX 454 pyrosequencing 560 m 5.8 ûC neg 20 ûC 34.9 psu