In accordance with SO #3423 - The Gulf of America and SO #3424 - Mount McKinley and Landmarks Honoring the Alaskan People, new USGS data releases specific to those named places will utilize the new name Gulf of America and the restored name Mount McKinley. Per USGS practice, historical data will retain the name of the geographic features as they were known at the time the data were originally released.

Data Release

Experimental PCR Data on Soil DNA Extracts

By Dale Griffin and Steven H. Douglas

USGS, St. Petersburg, Florida

Summary

Bacillus species and B. anthracis presence/absence data were determined in 4,770 soil samples collected across the contiguous United States in collaboration with the USEPA. PCR data for Bacillus species and B. anthracis rpoB gene PCR amplicon detection was reported as non-detect (n), low (l), medium (m), and high (h). Results for both pag and lef genes of the pX01 plasmid were reported by the University of South Florida's Center for Biological Defense. This data was recorded as negative or positive for each of the genes and included the following combinations: neg/neg, pos/neg, neg/pos, and pos/pos. Data for the pX02 plasmid were recorded as negative (blank) or positive (Y).

Data

File Name and Description Metadata (XML format) Metadata (text format) Download

banthracis_detection_survey_data.zip
Tabular data results for the rpoB gene PCR amplicon detection analysis of Bacillus species and B. anthracis. Supplementary data describe the number of samples screened in each state and the number and percent with positive Bacillus sp. detection (.csv, .xlsx)

banthracis_detection_survey_
metadata.xml

banthracis_detection_survey_
metadata.txt

banthracis_detection_survey_
data.zip

(203 KB)

Location map
Figure 1. Location map.

Suggested Citation

Griffin, D.W., and Douglas, S.H., 2016, Experimental PCR data on soil DNA extracts: U.S. Geological Survey data release, https://doi.org/10.5066/F7WW7FRJ.