Data Release

Cold-water Coral Metagenomes (Lophelia pertusa) From the Gulf of Mexico and Atlantic Ocean: Raw Data

By Christina A. Kellogg and Dawn B. Goldsmith

USGS, St. Petersburg, Florida

Summary

The files in this data release are the raw DNA metagenome sequence files referenced in Chapter 2 of the Bureau of Ocean Energy Management Environmental Studies Program Report entitled “Multidisciplinary Assessment of Deep-Water Coral Ecosystems: Tools to Detect Impacts of Sub-lethal Stress.” Chapter 2, written by Christina A. Kellogg, is entitled “Deep-Sea Coral Metagenomes.” The files represent a shotgun metagenome dataset from ten unique colonies of the cold-water coral Lophelia pertusa sampled in the Gulf of Mexico and western Atlantic Ocean to examine their microbial metagenomes. Samples from the Gulf of Mexico were collected in 2009 and 2010. Samples from the Atlantic Ocean were collected in 2009. The sequences were produced by random shearing of the DNA, construction of a TruSeq library, and sequencing by Argonne National Laboratory on an Illumina HiSeq 2000 sequencing system. The raw data files associated with this study have also been submitted to the NCBI Sequence Read Archive under Bioproject number PRJNA471489. For more information, please see the README file.

Kellogg, C.A., 2019, Deep-sea coral metagenomes, chap. 2 of Prouty, N.G., Kellogg, C.A., and Morrison, C.L., Multidisciplinary assessment of deepwater coral ecosystems—Tools to detect impacts of sub-lethal stress: Bureau of Ocean Energy Management, Gulf of Mexico OCS Region, OCS Study BOEM 2019–034, 114 p., https://espis.boem.gov/final%20reports/BOEM_2019-034.pdf. [Prepared under U.S. Geological Survey Outer Continental Shelf funding.]

Data

File Name and Description Metadata (XML format) Metadata (text format) Download File
Lophelia_metagenomes_raw_data_1.zip
Zip file containing raw data for CK1-CK6 (FASTQ, .csv), and MIMS sampling metadata.
Lophelia_metagenome_metadata.xml Lophelia_metagenome_metadata.txt Lophelia_metagenomes_raw_data_1.zip
(17.5 GB)
Lophelia_metagenomes_raw_data_2.zip
Zip file containing raw data for CK7-CK12, PhiX control (FASTQ, .csv), and MIMS sampling metadata.
Same as above Same as above Lophelia_metagenomes_raw_data_2.zip
(10.3 GB)
Supplemental information
README_Lophelia_metagenomes.txt
Readme file explaining data.
Not applicable Not applicable README_Lophelia_metagenomes.txt
(3 KB)

Graphic map showing locations of 4 sample sites with red triangles
Figure 1. Locations of sampling.

Suggested Citation

Kellogg, C.A., and Goldsmith, D.B., 2018, Cold-water coral metagenomes (Lophelia pertusa) from the Gulf of Mexico and Atlantic Ocean—raw data: U.S. Geological Survey data release, https://doi.org/10.5066/P9AA0H3B.