The files in this data release (https://doi.org/10.5066/P9LRIX9E) are those referenced in the journal article by Evans and others (2023, citation below) entitled "Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD)." The files contain an amplicon sequence variant (ASV) table and the raw 16S ribosomal ribonucleic acid (rRNA) gene amplicon deoxyribonucleic acid (DNA) files from fifty-five 0.22-micrometer (µm) size fractions of tangential flow filtration (TFF) concentrated microbial communities derived from mesocosms consisting of filtered seawater in which either healthy or diseased corals (Diploria labyrinthiformis, Pseudodiploria strigosa, Montastraea cavernosa, Colpophyllia natans, and Orbicella faveolata) had been incubated, as well as well as six reagent blanks, three mock communities, and a 0.22 µm-filtered ultraviolet (UV) treated seawater (FSW) control. 

Evans, J.S., Paul, V.J., Ushijima, B., Pitts, K.A., and Kellogg, C.A., 2023, Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD): PeerJ, v. 11, art. 15836, https://doi.org/10.7717/peerj15836.

Extraction of DNA from the samples and blanks occurred between February and May 2021 at the U.S. Geological Survey St. Petersburg Coastal and Marine Science Center (USGS SPCMSC) Coral Microbial Ecology Laboratory in St. Petersburg, FL, USA using the Qiagen DNeasy PowerBiofilm Kit. Library preparation and DNA sequencing were conducted on July 28th, 2021 by the Michigan State University RTSF Genomics Core (East Lansing, MI) using primers 515F: GTGCCAGCMGCCGCGGTAA and 806R: GGACTACHVGGGTWTCTAAT to target the V4 variable region of the 16S rRNA gene on a MiSeq sequencing system with v2 chemistry to obtain paired-end 250-bp reads. The raw data files associated with this data release have also been submitted to the NCBI Sequence Read Archive under Bioproject number PRJNA918331. For more information, please see the metadata files.

The file labeled "SCTLD_FullFilterSet_raw_data.zip" contains 110 raw 16S rRNA gene sequence files, 10 KitBlank extraction control sequence files, four mock community sequence files, two filtered seawater sequence files, four control sequence files provided by the sequencer, and an md5.txt file (designed to test the downloaded files for errors). The data consist of two compressed FASTQ sequence files (paired end forward and reverse reads) per sample (n= 55), and two compressed FASTQ sequence files per control (n= 10).

The files labeled "SCTLD_FullFilterSet_SRA_metadata.zip" and "SCTLD_FullFilterSet_MIMARKS.zip" contain metadata files (.csv, .txt, .xlsx) that are SRA and MIMARKS (minimum information about a marker sequence) compliant, based on standards developed by the Genomic Standards Consortium for reporting marker gene sequences (Yilmaz and others, 2011, Nature Biotechnology 29:415-420, doi:10.1038/nbt.1823). The column headers in these metadata files are defined below. An entry of "NA" is defined as "not applicable."

sample_name: A name chosen for the sample that is concise, unique, consistent, and as informative as possible.

sample_ID: Sample identifier within the sequence files.

sample_title: Title of sample.

bioproject_accession: The accession number of the BioProject(s) to which the BioSample belongs. A valid BioProject accession has prefix PRJN, PRJE or PRJD, e.g., PRJNA12345.

organism: The most descriptive organism name for this sample (to the species, if possible).

collection_date: The date on which the sample was collected. 

env_broad_scale: Terms that identify the major environment type(s) where your sample was collected. 

env_local_scale: Terms that identify environmental entities having causal influences upon the entity at time of sampling.

env_medium: Terms that identify the material displaced by the entity at time of sampling. Recommend subclasses of environmental material.

geo_loc_name: Geographical origin of the sample. Colon used to separate the country or ocean from more detailed information about the location, e.g. "Canada: Vancouver" or "Germany: halfway down Zugspitze, Alps"

lat_lon: The geographical coordinates of the location where the sample was collected. Specified as degrees latitude and longitude in the format "d[d.dddd] N|S d[dd.dddd] W|E", e.g., 38.98 N 77.11 W

samp_collect_device: Method or device employed for collecting sample.

samp_mat_process: Processing applied to the sample during or after isolation.

samp_store_loc: Name of location where sample was stored.

samp_store_temp: Temperature at which the sample was stored in degrees Celsius.

description: Description of the sample.

contact_name: Primary contact name.

contact_email: Primary contact email.

The file labeled "SCTLD_FullFilterSet_ASV_Table.zip" contains tabulations of amplicon sequence variants (ASVs), their DNA sequences, their taxonomic identification, and abundance for each sample and controls. The ASV table was produced using QIIME2 version 2022.2 (Bokulich and others, 2018; Robeson and others, 2021). The column headers in the SCTLD_FullFilterSet_ASV_Table data files (.csv, .txt, .xlsx) are defined as follows:

id: Amplicon sequence variant, unique identifier generated by QIIME2.

Sequence: DNA sequence.

Taxon: Taxonomy of the DNA sequence, identified to the most specific level.

Confidence: Calculated confidence in the taxonomy assignment generated by QIIME2.

BB-DL-1: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 µm-filtered seawater.

BB-DL-2: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 µm-filtered seawater.

BB-PS-1: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Cnat-15: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Cnat-20: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Cnat-23: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Cnat-8-4: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Cnat-M1: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Cnat-M2: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-16: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-17: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-18: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-19: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-20: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-21: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-23: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-24: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-25: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-8: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnD-9: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnH-100: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnH-101-Oct: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnH-104: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

CnH-106: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Dlab-2-3-PQ: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Dlab-3: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 µm-filtered seawater.

DLD-1: Column headers represent sample names. Microbial 16S community shed by diseased Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 µm-filtered seawater.

FSW: Column headers represent sample names. Microbial 16S sequence library of UV-treated, 0.2 µm-filtered seawater.

FtLMcD-30-31: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

kit_blank: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

kitblank03May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

Kitblank04May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

kitblank05May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

kitblank06May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

Mcav-17: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Mcav-18: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Mcav-8: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Mcav-M1: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Mcav-M3: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

McD-56: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

McD-57: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

McD-58: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

McH-101: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

McH-106-4: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

mock: Column headers represent sample names. ABRF-MGRG 10 Strain Even Mix Genomic Material (ATCC MSA-3001).

mock2: Column headers represent sample names. ABRF-MGRG 10 Strain Even Mix Genomic Material (ATCC MSA-3001).

Ofav-16: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Ofav-19: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.
 
Ofav-26: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Ofav-9: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.

OFH-100: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.

OFH-101: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.

OFH-104: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.

OFH-105-3: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 µm-filtered seawater.

PsD-5: Column headers represent sample names. Microbial 16S community shed by diseased Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Pstr-10: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Pstr-4: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

Pstr-5: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 µm-filtered seawater.

ZymoMockDNA: Column headers represent sample names. Microbial 16S community of the sequencer-provided mock community.

ASV ID: Numeric ASV identification.

The file labeled "SCTLD_FullFilterSet_ASV_Table_Eukaryota.zip" contains tabulations of amplicon sequence variants (ASVs) prior to the removal of chloroplasts and mitochondria, their DNA sequences, their taxonomic identification, and abundance for each sample and controls. The ASV table was produced using QIIME2 version 2022.2 (Bolyen and others, 2019, Nature Biotechnology 37:852-857, doi: 10.1038/s41587-019-0209-9). The column headers in the SCTLD_FullFilterSet_ASV_Table_Eukaryota data files (.csv, .txt, .xlsx) are defined as follows:

id: amplicon sequence variant, unique identifier generated by QIIME2.

Sequence: DNA sequence.

Taxon: Taxonomy of the DNA sequence, identified to the most specific level.

Confidence: calculated confidence in the taxonomy assignment generated by QIIME2

BB-DL-1: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 μm-filtered seawater.

BB-DL-2: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 μm-filtered seawater.

BB-PS-1: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Cnat-15: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Cnat-20: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Cnat-23: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Cnat-8-4: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Cnat-M1: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Cnat-M2: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-16: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-17: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-18: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-19: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-20: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-21: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-23: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-24: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-25: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-8: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnD-9: Column headers represent sample names. Microbial 16S community shed by diseased Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnH-100: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnH-101-Oct: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnH-104: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

CnH-106: Column headers represent sample names. Microbial 16S community shed by healthy Colpophyllia natans into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Dlab-2-3-PQ: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Dlab-3: Column headers represent sample names. Microbial 16S community shed by healthy Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 μm-filtered seawater.

DLD-1: Column headers represent sample names. Microbial 16S community shed by diseased Diploria labyrinthiformis into mesocosm of UV-treated, 0.2 μm-filtered seawater.

FSW: Column headers represent sample names. Microbial 16S sequence library of UV-treated, 0.2 μm-filtered seawater.

FtLMcD-30-31: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

kit_blank: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

kitblank03May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

Kitblank04May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

kitblank05May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

kitblank06May: Column headers represent sample names. Microbial 16S sequence library of Qiagen Dneasy PowerBiofilm extraction kit blank.

Mcav-17: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Mcav-18: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Mcav-8: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Mcav-M1: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Mcav-M3: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

McD-56: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

McD-57: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

McD-58: Column headers represent sample names. Microbial 16S community shed by diseased Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

McH-101: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

McH-106-4: Column headers represent sample names. Microbial 16S community shed by healthy Montastraea cavernosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

mock: Column headers represent sample names. ABRF-MGRG 10 Strain Even Mix Genomic Material (ATCC MSA-3001).

mock2: Column headers represent sample names. ABRF-MGRG 10 Strain Even Mix Genomic Material (ATCC MSA-3001).

Ofav-16: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Ofav-19: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.
 
Ofav-26: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Ofav-9: Column headers represent sample names. Microbial 16S community shed by diseased Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.

OFH-100: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.

OFH-101: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.

OFH-104: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.

OFH-105-3: Column headers represent sample names. Microbial 16S community shed by healthy Orbicella faveolata into mesocosm of UV-treated, 0.2 μm-filtered seawater.

PsD-5: Column headers represent sample names. Microbial 16S community shed by diseased Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Pstr-10: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Pstr-4: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

Pstr-5: Column headers represent sample names. Microbial 16S community shed by healthy Pseudodiploria strigosa into mesocosm of UV-treated, 0.2 μm-filtered seawater.

ZymoMockDNA: Column headers represent sample names. Microbial 16S community of the sequencer-provided mock community.


The file labeled "SCTLD_FullFilterSet_TFF_Filtrates.zip" contains a table indicating the volume of liquid that remained unfiltered after TFF and five hours of 0.025 μm pore size filtration, sorted by experiment (Run 1 - October 2019; Run 2 - November 2020; and Run 3 - March 2021) and mesocosm type (diseased or healthy) and coral sample ID used as microbial donors. The column headers in the SCTLD_FullFilterSet_TFF_Filtrates data files (.csv, .txt, .xlsx) are defined as follows:

Experimental Run: Represents the run number, and the month and year the run took place.

Donor Coral Health: Represents the apparent health (diseased or healthy) of the coral used as the source of microbes incubated in the mesocosm.

Donor Coral ID: Represents the sample names of the coral used as the source of microbes incubated in the mesocosm.

Donor Coral Species: Represents the species of coral incubated in the mesocosm (CNAT =Colpophyllia natans, DLAB =Diploria labyrinthiformis, MCAV =Montastraea cavernosa, OANN =Orbicella annularis, OFAV =Orbicella faveolata, PSTR =Pseudodiploria strigosa).

Final TFF Concentrate Volume (mL): Represents the remaining volume of liquid from each mesocosm following tangential flow filtration, but prior to size fractionation.

Approx. Volume Remaining After 5h of 0.025 μm Filtration: Represents the volume of 0.22 μm liquid filtrate from each mesocosm remaining unfiltered through a 0.025 μm pore size filter after five hours of filtration. A “0” indicates that the entire volume was filtered through the 0.025 μm filter, so no 0.22 μm pore size filtrate remained after 5 hours, and only filters were applied to receiver corals during the transmission experiments.


The file labeled "SCTLD_FullFilterSet_SCTLD_bioindicators.zip" shows the amplicon sequence variants ASVs detected in this study that represent 100% sequence identity matches for the overlapping region of SCTLD bioindicator ASVs (Becker and others, 2021, Environmental Microbiology 24(3), doi: 10.1111/1462-2920.15718) from the U.S. Virgin Islands and disease-associated ASVs from Florida SCTLD studies. The column headers in the SCTLD_FullFilterSet_SCTLD_bioindicators data files (.csv, .txt, .xlsx) are defined as follows:

SCTLD Bioindicator ASV IDs (Becker et al., 2021): Provided ASV identification for ASVs previously identified by Becker and others,2021 as SCTLD bioindicators ASV identification.

Sequence (from Becker et al., 2021): Sequences associated with the provided SCTLD bioindicator ASV identification. 

corresponding ASV ID, this study: ASV identifications from this study representing 100% sequence identity matches for the overlapping region of the SCTLD bioindicator ASV sequences.

Sequence, this study: Sequences associated with this study's ASV identifications.


The file labeled "SCTLD_FullFilterSet_ANCOM_results.zip" contains direct outputs from the differential abundance analysis (ANCOM) analysis plugin (QIIME2 version 2022.2). The column headers in the SCTLD_FullFilter_Set_ANCOM_results data files (.csv, .txt, .xlsx) are defined as follows:

Sequence ID: Individual sequence identifications produced by the program.

W: The number of times the null hypothesis was rejected per ASV sequence.

Reject null hypothesis: TRUE or FALSE values, indicating whether the null hypothesis has been rejected or not.

Group: The sequence ID associated with each of the percentile values thereafter.

Diseased (0 percentile): The minimum number of reads observed in the corresponding group of diseased samples.

Diseased (25 percentile): The maximum number of reads observed in the corresponding group that appear in 25% of diseased samples.

Diseased (50 percentile): The maximum number of reads observed in the corresponding group that appear in 50% of diseased samples.

Diseased (75 percentile): The maximum number of reads observed in the corresponding group that appear in 75% of diseased samples.

Diseased (100 percentile): The maximum number of reads observed in the corresponding group that appear in 100% of diseased samples.

Healthy (0 percentile): The minimum number of reads observed in the corresponding group of apparently healthy samples.

Healthy (25 percentile): The maximum number of reads observed in the corresponding group that appear in 25% of apparently healthy samples.

Healthy (50 percentile): The maximum number of reads observed in the corresponding group that appear in 50% of apparently healthy samples.

Healthy (75 percentile): The maximum number of reads observed in the corresponding group that appear in 75% of apparently healthy samples.

Healthy (100 percentile): The maximum number of reads observed in the corresponding group that appear in 100% of apparently healthy samples.