Metadata:
  Identification_Information:
    Citation:
      Citation_Information:
        Originator: Gallery, Dominique N.
        Originator: Kuffner, Ilsa B.
        Originator: Toth, Lauren T.
        Publication_Date: 20210430
        Title: DNA microsatellite markers for Mustard Hill Coral (Porites astreoides) from the Florida Keys Reef Tract
        Geospatial_Data_Presentation_Form: tabular digital data
        Larger_Work_Citation:
          Citation_Information:
            Originator: Gallery, Dominique N.
            Originator: Kuffner, Ilsa B.
            Originator: Toth, Lauren T.
            Publication_Date: 20210430
            Title: DNA microsatellite markers for Mustard Hill Coral (Porites astreoides) from the Florida Keys Reef Tract
            Series_Information:
              Series_Name: U.S. Geological Survey data release
              Issue_Identification: doi:10.5066/P9R8NZ2J
            Publication_Information:
              Publication_Place: St. Petersburg, Florida
              Publisher: U.S. Geological Survey - St. Petersburg Coastal and Marine Science Center
            Online_Linkage: https://doi.org/10.5066/P9R8NZ2J
    Description:
      Abstract: This data set includes: (1) allele sizes of 11 previously published microsatellites (Kenkel and others, 2013; Shearer and Coffroth, 2004) for 39 individuals of mustard hill coral, Porites astreoides (mustard hill coralP. astreoides) collected in the Spring of 2017 from four locations in the Florida Keys Reef Tract (FKRT): Fowey Rocks, Crocker Reef, Sombrero Reef and Pulaski Shoal and (2) the deoxyribonucleic acid (DNA) concentration of the extracted DNA prior to polymerase chain reaction (PCR) reactions for the samples. These data were used in Gallery and others (2021) to evaluate the genetic diversity and connectivity of individuals among and between sites.
      Purpose: Microsatellites were used to determine the genetic relatedness of the sampled Porites astreoides in the Florida Keys.
    Time_Period_of_Content:
      Time_Period_Information:
        Multiple_Dates/Times:
          Single_Date/Time:
            Calendar_Date: 201705
          Single_Date/Time:
            Calendar_Date: 201909
          Single_Date/Time:
            Calendar_Date: 201908
          Single_Date/Time:
            Calendar_Date: 201911
      Currentness_Reference: Date of process steps
    Status:
      Progress: Complete
      Maintenance_and_Update_Frequency: None planned
    Spatial_Domain:
      Bounding_Coordinates:
        West_Bounding_Coordinate: -82.77280
        East_Bounding_Coordinate: -80.09560
        North_Bounding_Coordinate: 25.59047
        South_Bounding_Coordinate: 24.62687
    Keywords:
      Theme:
        Theme_Keyword_Thesaurus: USGS Metadata Identifier
        Theme_Keyword: USGS:99036c02-ead0-4f81-aa00-cc32f825f223
      Theme:
        Theme_Keyword_Thesaurus: USGS Thesaurus
        Theme_Keyword: marine biology
        Theme_Keyword: genetic diversity
        Theme_Keyword: genotype
        Theme_Keyword: population and community ecology
        Theme_Keyword: population dynamics
        Theme_Keyword: molecular biology
        Theme_Keyword: coelenterates
      Place:
        Place_Keyword_Thesaurus: None
        Place_Keyword: Florida Keys
        Place_Keyword: Pulaski Shoal
        Place_Keyword: Sombrero Reef
        Place_Keyword: Crocker Reef
        Place_Keyword: Fowey Rocks
    Access_Constraints: none
    Use_Constraints: none
    Point_of_Contact:
      Contact_Information:
        Contact_Person_Primary:
          Contact_Person: Lauren T. Toth
          Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
        Contact_Position: Physical Scientist
        Contact_Address:
          Address_Type: mailing and physical
          Address: 600 4Th Street South
          City: St. Petersburg
          State_or_Province: Florida
          Postal_Code: 33701
          Country: United States
        Contact_Voice_Telephone: 727-502-8029
        Contact_Electronic_Mail_Address: ltoth@usgs.gov
    Cross_Reference:
      Citation_Information:
        Originator: D.N. Gallery
        Originator: M.L. Green
        Originator: I.B. Kuffner
        Originator: E.A. Lenz
        Originator: L.T. Toth
        Publication_Date: 20210709
        Title: Genetic structure and diversity of the mustard hill coral Porites astreoides along the Florida Keys reef tract
        Publication_Information:
          Publication_Place: Berlin, Germany
          Publisher: Marine Biodiversity, Springer Nature
        Online_Linkage: https://doi.org/10.1007/s12526-021-01196-7
    Cross_Reference:
      Citation_Information:
        Originator: C.D. Kenkel
        Originator: G. Goodbody-Gringley
        Originator: D. Caillaud
        Originator: S.W. Davies
        Originator: E. Bartels
        Originator: M.V. Matz
        Publication_Date: 20130730
        Title: Evidence for a host role in thermotolerance divergence between populations of the mustard hill coral (Porites astreoides) from different reef environments
        Publication_Information:
          Publication_Place: Hoboken, New Jersey
          Publisher: Molecular Ecology, Wiley
        Online_Linkage: https://doi.org/10.1111/mec.12391
    Cross_Reference:
      Citation_Information:
        Originator: T.L. Shearer
        Originator: M.A. Coffroth
        Publication_Date: 20040608
        Title: Isolation of microsatellite loci from the scleractinian corals, Montastraea cavernosa and Porites astreoides
        Publication_Information:
          Publication_Place: Hoboken, New Jersey
          Publisher: Molecular Ecology Notes, Wiley
        Online_Linkage: https://doi.org/10.1111/j.1471-8286.2004.00653.x
    Cross_Reference:
      Citation_Information:
        Originator: C. van Oosterhout
        Originator: W.F. Hutchinson
        Originator: D.P.M. Wills
        Originator: P. Shipley
        Publication_Date: 20040608
        Title: Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data
        Publication_Information:
          Publication_Place: Hoboken, New Jersey
          Publisher: Molecular Ecology Notes, Wiley
        Online_Linkage: https://doi.org/10.1111/j.1471-8286.2004.00684.x
    Cross_Reference:
      Citation_Information:
        Originator: S.W. Davies
        Originator: M. Rahman
        Originator: E. Meyer
        Originator: E.A. Green
        Originator: E. Buschiazzo
        Originator: M. Medina
        Originator: M.V. Matz
        Publication_Date: 20121031
        Title: Novel polymorphic microsatellite markers for population genetics of the endangered Caribbean star coral, Montastraea faveolata
        Publication_Information:
          Publication_Place: Berlin, Germany
          Publisher: Marine Biodiversity, Springer
        Online_Linkage: https://doi.org/10.1007/s12526-012-0133-4
    Cross_Reference:
      Citation_Information:
        Originator: A. Baker
        Originator: R. Cunning
        Publication_Date: 20160304
        Title: Bulk gDNA extraction from coral samples
        Publication_Information:
          Publication_Place: Online
          Publisher: protocols.io
        Online_Linkage: https://doi.org/10.17504/protocols.io.dyq7vv
    Cross_Reference:
      Citation_Information:
        Originator: I.B. Kuffner
        Originator: T.D. Hickey
        Originator: J.M. Morrison
        Publication_Date: 20130608
        Title: Calcification rates of the massive coral Siderastrea siderea and crustose coralline algae along the Florida Keys (USA) outer-reef tract
        Publication_Information:
          Publication_Place: Berlin, Germany
          Publisher: Coral Reefs, Springer
        Online_Linkage: https://doi.org/10.1007/s00338-013-1047-8
    Cross_Reference:
      Citation_Information:
        Originator: J.M. Morrison
        Originator: I.B. Kuffner
        Originator: T.D. Hickey
        Publication_Date: 20130730
        Title: Methods for monitoring corals and crustose coralline algae to quantify in-situ calcification rates
        Publication_Information:
          Publication_Place: Reston, VA
          Publisher: U.S. Geological Survey
        Online_Linkage: http://pubs.usgs.gov/of/2013/1159/
    Cross_Reference:
      Citation_Information:
        Originator: E.A. Lenz
        Originator: L.A. Bartlett
        Originator: A. Stathakopoulos
        Originator: I.B. Kuffner
        Publication_Date: 20210622
        Title: Physiological differences in bleaching response of the coral Porites astreoides along the Florida Keys reef tract during high-temperature stress
        Publication_Information:
          Publication_Place: Lausanne, Switzerland
          Publisher: Frontiers in Marine Science, Frontiers
        Online_Linkage: https://doi.org/10.3389/fmars.2021.615795
  Data_Quality_Information:
    Attribute_Accuracy:
      Attribute_Accuracy_Report: Individual sample ID's were cross-checked and verified in Microsoft Excel Version 2002 worksheets for extraction, PCR, and placement into plates sent for data analysis. For quality control, all samples were run on an agarose gel to ensure DNA extraction was successful prior to running PCR. Microsatellite marker calls were processed through Micro-Checker V. 2.2.3 (van Oosterhout and others 2004) to detect errors in data interpretation.
    Logical_Consistency_Report: Reported allele sizes were reviewed to confirm they conform to expected distributions using Micro-Checker V. 2.2.3 (van Oosterhout and others 2004).
    Completeness_Report: Any data omissions for entities are explained in the Entity and Attribute definition section below. Blank data indicates data was homozygous for that allele. Attribute data equaling “NP” in a cell of the data set refers to a sample that did not amplify or show any product (“no product”). Attribute data equaling "?" refers to a sample with product not sufficient (DNA concentration too low) to call the allele.
    Positional_Accuracy:
      Horizontal_Positional_Accuracy:
        Horizontal_Positional_Accuracy_Report: No formal positional accuracy tests were conducted
      Vertical_Positional_Accuracy:
        Vertical_Positional_Accuracy_Report: No formal positional accuracy tests were conducted
    Lineage:
      Process_Step:
        Process_Description: Porites astreoides samples were obtained from four locations along the FKRT using a Global Positioning System (GPS): Fowey Rocks in Biscayne National Park (25.59047, -80.09560), Crocker Reef in the Upper Florida Keys (24.90908, -80.52665), Sombrero Reef in the Middle Florida Keys (24.62687, -81.10893), and Pulaski Shoal in the Dry Tortugas National Park (24.69355, -82.77280). The corals were collected within a 100 to 200 square meter area at each site, and colonies selected for sampling were no closer than approximately 20 meters (Lenz and others, 2021; compare with Kuffner and others, 2013). After collection, the corals were grown for two years (from 2015 to 2017) on cinderblocks installed on the reef as part of the U.S. Geological Survey calcification assessment project (Kuffner and others 2013; Morrison and others 2013). Coral colony condition was assessed, and growth rates were monitored every six months during the study using the methods outlined in Kuffner and others (2013; data presented in Lenz and others, 2021). At the end of the two-year period, several 4 millimeter thick slabs were cut from the center of the colonies. One slab was used for host tissue sampling in this study as well as symbiont sampling and histological examination in an associated study (Lenz and others, 2021). The rest of the colonies were outplanted onto the reefs.
        Process_Date: 201705
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Ilsa B.Kuffner
              Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
            Contact_Address:
              Address_Type: mailing and physical
              Address: 600 4th Street South
              City: St. Petersburg
              State_or_Province: Florida
              Postal_Code: 33701
              Country: United States
            Contact_Voice_Telephone: 727-502-8048
            Contact_Electronic_Mail_Address: ikuffner@usgs.gov
      Process_Step:
        Process_Description: DNA Extraction: Preparation of cetyltrimethylammonium bromide (CTAB) mixture (Baker and Cunning 2016).
        Process_Date: 201905
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Dominique N. Gallery
              Contact_Organization: University of South Florida, U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
            Contact_Address:
              Address_Type: mailing and physical
              Address: 600 4th Street South
              City: St. Petersburg
              State_or_Province: Florida
              Postal_Code: 33701
              Country: United States
            Contact_Voice_Telephone: 727-502-8000
            Contact_Electronic_Mail_Address: dgallery@mail.usf.edu
      Process_Step:
        Process_Description: Quibit 3.0 analysis to quantify DNA extracted.
        Process_Date: 201905
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Dominique N. Gallery
              Contact_Organization: University of South Florida, U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
            Contact_Address:
              Address_Type: mailing and physical
              Address: 600 4th Street South
              City: St. Petersburg
              State_or_Province: Florida
              Postal_Code: 33701
              Country: United States
            Contact_Voice_Telephone: 727-502-8000
            Contact_Electronic_Mail_Address: dgallery@mail.usf.edu
      Process_Step:
        Process_Description: Run electrophoresis gel using a 1.5% agarose gel suspension to visualize DNA extraction.
        Process_Date: 201905
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Dominique N. Gallery
              Contact_Organization: University of South Florida, U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
            Contact_Address:
              Address_Type: mailing and physical
              Address: 600 4th Street South
              City: St. Petersburg
              State_or_Province: Florida
              Postal_Code: 33701
              Country: United States
            Contact_Voice_Telephone: 727-502-8000
            Contact_Electronic_Mail_Address: dgallery@mail.usf.edu
      Process_Step:
        Process_Description: Amplified DNA using PCR in 10 microliter (μL) reactions with 10 nanograms (ng) DNA, 1 micromole (μM) fluorescently-labeled forward primer, 1 μM reverse primer, 0.2 millimole (mM) deoxynucleoside triphosphate (dNTP) (Promega), 2μL 5 times (5x) PCR GoTaq Flexi buffer (Promega), 0.25 units (0.05 μL) GoTaq Flexi polymerase (Promega), 2 mM MgCl2 (Promega). The PCR procedure was based on the protocols of Davies and others (2013), but was modified as follows: (1) increased to 1 μM fluorescently-labeled forward primer and 1 μM reverse primer, (2) modified to 2 μL 5X PCR GoTaq Flexi buffer (Promega). Increased 0.25 U GoTaq Flexi polymerase (Promega), (3) added 2 mM  magnesium chloride (MgCl2) (Promega), and (4) thermocycler protocol modifications: Increased initial denaturing temperature 95 degrees Celsius (˚C). Increased number of cycles to 40 at (95˚C for 40 seconds, 58–60˚C for 60 seconds, 72˚C for 60 seconds).
        Process_Date: 201908
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Dominique N. Gallery
              Contact_Organization: University of South Florida, U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
            Contact_Address:
              Address_Type: mailing and physical
              Address: 600 4th Street South
              City: St. Petersburg
              State_or_Province: Florida
              Postal_Code: 33701
              Country: United States
            Contact_Voice_Telephone: 727-502-8000
            Contact_Electronic_Mail_Address: dgallery@mail.usf.edu
      Process_Step:
        Process_Description: Sent select samples to the University of Illinois Urbana-Champagne (UIUC) Core Sequencing Facility for fragment analysis of dilution sets to determine optimum dilution for each primer set.
        Process_Date: 201908
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Unknown
              Contact_Organization: University of Illinois Urbana-Champagne Core Sequencing Facility
            Contact_Address:
              Address_Type: mailing and physical
              Address: 334 Edward R. Madigan Laboratory, 1201 W. Gregory Drive
              City: Urbana
              State_or_Province: Illinois
              Postal_Code: 61801
              Country: United States
            Contact_Voice_Telephone: 217-333-9520
            Contact_Electronic_Mail_Address: dna-seq@illinois.edu
      Process_Step:
        Process_Description: Sent samples to the UIUC Core Sequencing Facility for fragment analysis using Applied Biosystems 3730x1 DNA Analyzer and ROX1000 standards.
        Process_Date: 201909
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Unknown
              Contact_Organization: University of Illinois Urbana-Champagne Core Sequencing Facility
            Contact_Address:
              Address_Type: mailing and physical
              Address: 334 Edward R. Madigan Laboratory, 1201 W. Gregory Drive
              City: Urbana
              State_or_Province: Illinois
              Postal_Code: 61801
              Country: United States
            Contact_Voice_Telephone: 217-333-9520
            Contact_Electronic_Mail_Address: dna-seq@illinois.edu
      Process_Step:
        Process_Description: USGS researchers worked with collaborators at University of South Florida (USF), Saint Petersburg to analyze microsatellites using GeneMapper 5.0 to determine microsatellite calls. Micro-Checker (version 2.2.3) was used to confirm that calls were accurate (for example, correct number of bases between calls). Allele bins were assigned by rounding observed size to the closest integer, except when Micro-Checker indicated that the bins should be adjusted due to errors with nucleotide repeats (for example, only 2 base pairs [bp] difference for trinucleotide repeats). In those cases, alleles were marked as unknown "?" instead of called.
        Process_Date: 201910
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Dominique N. Gallery
              Contact_Organization: University of South Florida, U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
            Contact_Address:
              Address_Type: mailing and physical
              Address: 600 4th Street South
              City: St. Petersburg
              State_or_Province: Florida
              Postal_Code: 33701
              Country: United States
            Contact_Voice_Telephone: 727-502-8000
            Contact_Electronic_Mail_Address: dgallery@mail.usf.edu
      Process_Step:
        Process_Description: Reran PCR for samples that failed to amplify well or indicated potential null alleles.
        Process_Date: 201911
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Dominique N. Gallery
              Contact_Organization: University of South Florida, U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
            Contact_Address:
              Address_Type: mailing and physical
              Address: 600 4th Street South
              City: St. Petersburg
              State_or_Province: Florida
              Postal_Code: 33701
              Country: United States
            Contact_Voice_Telephone: 727-502-8000
            Contact_Electronic_Mail_Address: dgallery@mail.usf.edu
      Process_Step:
        Process_Description: Sent rerun samples to the UIUC Core Sequencing Facility for fragment analysis using Applied Biosystems 3730x1 DNA Analyzer and ROX1000 standards. "GeneScan™ 1000 ROX™ Size Standard is an internal lane size standard developed for use with Applied Biosystems® fluorescence-based DNA electrophoresis systems. The use of an internal lane size standard during electrophoresis enables automated data analysis and precise DNA fragment size comparisons between electrophoresis runs. GeneScan™ 1000 ROX™ Size Standard is designed for sizing DNA fragments in the 47–946 bp range and provides labeled fragments of 47, 51, 55, 82, 85 93 99, 126, 136, 262, 293, 317, 439, 557, 692, 695 and 946 bases. Each of the DNA fragments is labeled with ROX™ fluorophore, which results in a single peak when run under non-denaturing conditions." Quotation is from the ThermoFisher Catalog # 401098
        Process_Date: 201911
        Process_Contact:
          Contact_Information:
            Contact_Person_Primary:
              Contact_Person: Unknown
              Contact_Organization: University of Illinois Urbana-Champagne Core Sequencing Facility
            Contact_Address:
              Address_Type: mailing and physical
              Address: 334 Edward R. Madigan Laboratory, 1201 W. Gregory Drive
              City: Urbana
              State_or_Province: Illinois
              Postal_Code: 61801
              Country: United States
            Contact_Voice_Telephone: 217-333-9520
            Contact_Electronic_Mail_Address: dna-seq@illinois.edu
  Spatial_Data_Organization_Information:
    Indirect_Spatial_Reference: GPS coordinates collected at the four study sites, Fowey Rocks, Crocker Reef, Sombrero Reef, Pulaski Shoal Light
    Direct_Spatial_Reference_Method: Point
  Spatial_Reference_Information:
    Horizontal_Coordinate_System_Definition:
      Geographic:
        Latitude_Resolution: 0.0197864699
        Longitude_Resolution: 0.0228595953
        Geographic_Coordinate_Units: Decimal degrees
      Geodetic_Model:
        Horizontal_Datum_Name: D_WGS_1984
        Ellipsoid_Name: WGS_1984
        Semi-major_Axis: 6378137.0
        Denominator_of_Flattening_Ratio: 298.257223563
  Entity_and_Attribute_Information:
    Detailed_Description:
      Entity_Type:
        Entity_Type_Label: Porites astreoides Microsatellite Data.csv, Porites astreoides Microsatellite Data.xlsx
        Entity_Type_Definition: Microsoft Excel comma-separated values (.csv) and worksheet (.xlsx) files containing data for the Microsatellite markers for Porites astreoides
        Entity_Type_Definition_Source: Authors
      Attribute:
        Attribute_Label: Sample Name
        Attribute_Definition: This is a U.S. Geological Survey, Coastal and Marine Science Center numeric or alphanumeric label that uniquely identifies each sample. Three numbers identify the sample. Negative controls use the identifier "NEG".
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Unrepresentable_Domain: A unique identifier given tofor each sample. A three-digit number was given to a tested sample. Negative controls were given the alphanumeric identifier "NEGP#".
      Attribute:
        Attribute_Label: Sample Type
        Attribute_Definition: Identification of sample or negative control.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Sample
            Enumerated_Domain_Value_Definition: Sample of P. astreoides
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Negative Control
            Enumerated_Domain_Value_Definition: Negative controls were utilized to ensure there was no cross-contamination during PCR.
            Enumerated_Domain_Value_Definition_Source: USGS
      Attribute:
        Attribute_Label: Sample Location
        Attribute_Definition: The study site at which the sample was collected.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: PLS
            Enumerated_Domain_Value_Definition: Pulaski Shoal
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: SMK
            Enumerated_Domain_Value_Definition: Sombrero Reef
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: FWY
            Enumerated_Domain_Value_Definition: Fowey Rocks
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: CRK
            Enumerated_Domain_Value_Definition: Crocker Reef
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Negative
            Enumerated_Domain_Value_Definition: Negative control has no location of origin.
            Enumerated_Domain_Value_Definition_Source: USGS
      Attribute:
        Attribute_Label: Panel
        Attribute_Definition: Microsatellite loci used for PCR amplification. Primers that begin with "Past_" are published in Kenkel and others (2013). Primers that begin with "PaGA" are published in Shearer and Coffroth (2004).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_2
            Enumerated_Domain_Value_Definition: Primer "Past_2" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_3
            Enumerated_Domain_Value_Definition: Primer "Past_3" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: PaGA7
            Enumerated_Domain_Value_Definition: Primer "PaGA7" as published in Shearer and Coffroth (2004)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_8
            Enumerated_Domain_Value_Definition: Primer "Past_8" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_10
            Enumerated_Domain_Value_Definition: Primer "Past_10" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_13
            Enumerated_Domain_Value_Definition: Primer "Past_13" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_16
            Enumerated_Domain_Value_Definition: Primer "Past_16" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_17
            Enumerated_Domain_Value_Definition: Primer "Past_17" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: Past_21
            Enumerated_Domain_Value_Definition: Primer "Past_21" as published in Kenkel and others (2013)
            Enumerated_Domain_Value_Definition_Source: Kenkel and others (2013)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: PaGA24
            Enumerated_Domain_Value_Definition: Primer "PaGA24" as published in Shearer and Coffroth (2004)
            Enumerated_Domain_Value_Definition_Source: Shearer and Coffroth (2004)
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: PaGA63
            Enumerated_Domain_Value_Definition: Primer "PaGA63" as published in Shearer and Coffroth (2004)
            Enumerated_Domain_Value_Definition_Source: Shearer and Coffroth (2004)
      Attribute:
        Attribute_Label: Run Date
        Attribute_Definition: The date the run was processed by UIUC Core Sequencing Facility.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 08/15/2019
            Range_Domain_Maximum: 11/13/2019
            Attribute_Units_of_Measure: Dates in MM/DD/YYYY format
      Attribute:
        Attribute_Label: Allele 1
        Attribute_Definition: The first allele called from Gene-Mapper program. An integer value for called alleles. "NP" indicates there was no product visible in Gene-Mapper to call. "?" indicates there was product visible, but the result was not strong enough to definitively call the allele.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 139
            Range_Domain_Maximum: 463
            Attribute_Units_of_Measure: Base pairs
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: NP
            Enumerated_Domain_Value_Definition: NP indicates there was no product visible in Gene-Mapper to call.
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: ?
            Enumerated_Domain_Value_Definition: "?" indicates there was product visible, but the result was not strong enough to definitively call the allele.
            Enumerated_Domain_Value_Definition_Source: USGS
      Attribute:
        Attribute_Label: Allele 2
        Attribute_Definition: The second allele called from Gene-Mapper program. An integer value for called alleles. "NP" indicates there was no product visible in Gene-Mapper to call. "?" indicates there was product visible, but the result was not strong enough to definitively call the allele. Homozygous alleles have the same value from Allele 1 repeated in Allele 2.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 141
            Range_Domain_Maximum: 463
            Attribute_Units_of_Measure: Base pairs
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: NP
            Enumerated_Domain_Value_Definition: Indicates there was no product visible in Gene-Mapper to call.
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: ?
            Enumerated_Domain_Value_Definition: ? indicates there was product visible, but the result was not strong enough to definitively call the allele.
            Enumerated_Domain_Value_Definition_Source: USGS
      Attribute:
        Attribute_Label: Size 1
        Attribute_Definition: A non-integer number that is the exact number of base pairs determined by Gene-Mapper based on Rox1000 dye size standard. NP indicates there was no product visible in Gene-Mapper.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 139.02
            Range_Domain_Maximum: 462.80
            Attribute_Units_of_Measure: Base pairs
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: NP
            Enumerated_Domain_Value_Definition: Indicates there was no product visible in Gene-Mapper to call.
            Enumerated_Domain_Value_Definition_Source: USGS
      Attribute:
        Attribute_Label: Size 2
        Attribute_Definition: A non-integer number that is the exact number of base pairs determined by Gene-Mapper based on Rox1000 dye size standard. NP indicates there was no product visible in Gene-Mapper. Homozygous alleles have the same value from Size 1 repeated in Size 2
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 141.18
            Range_Domain_Maximum: 463.80
            Attribute_Units_of_Measure: Base pairs
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: NP
            Enumerated_Domain_Value_Definition: NP indicates there was no product visible in Gene-Mapper.
            Enumerated_Domain_Value_Definition_Source: USGS
    Detailed_Description:
      Entity_Type:
        Entity_Type_Label: Porites_astreoides_DNA_concentration.csv, Porites_astreoides_DNA_concentration.xlsx
        Entity_Type_Definition: Microsoft Excel comma-separated values (.csv) and worksheet (.xlsx) files containing data for the concentration of DNA from Porites astreoides samples as determined by Qubit 3.0. These values were used to determine the volume of DNA added to PCR.
        Entity_Type_Definition_Source: USGS
      Attribute:
        Attribute_Label: Run Date
        Attribute_Definition: The date the run was processed by USGS researchers.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 05/5/2019
            Range_Domain_Maximum: 05/15/2019
            Attribute_Units_of_Measure: Dates in MM/DD/YYYY format
      Attribute:
        Attribute_Label: Test Name
        Attribute_Definition: Unique alpha-numeric identifier assigned to each sample by Qubit 3.0
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: Sample_#190505-174736
            Range_Domain_Maximum: Sample_#190515-170555
            Attribute_Units_of_Measure: N/A
      Attribute:
        Attribute_Label: Sample Number
        Attribute_Definition: This is a U.S. Geological Survey, Coastal and Marine Science Center alphanumeric label that uniquely identifies each sample. Three numbers identify the sample.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 207
            Range_Domain_Maximum: 497
            Attribute_Units_of_Measure: N/A
      Attribute:
        Attribute_Label: Sample Location
        Attribute_Definition: The is the study site at which the sample was collected.
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: CRK
            Enumerated_Domain_Value_Definition: Crocker Reef
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: FWY
            Enumerated_Domain_Value_Definition: Fowey Rocks
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: SMK
            Enumerated_Domain_Value_Definition: Sombrero Reef
            Enumerated_Domain_Value_Definition_Source: USGS
        Attribute_Domain_Values:
          Enumerated_Domain:
            Enumerated_Domain_Value: PLS
            Enumerated_Domain_Value_Definition: Pulaski Shoal
            Enumerated_Domain_Value_Definition_Source: USGS
      Attribute:
        Attribute_Label: Qubit tube concentration (ng/mL)
        Attribute_Definition: The concentration of double-stranded DNA in the assay tube in nanograms per milliliter (ng/mL).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.93
            Range_Domain_Maximum: 495
            Attribute_Units_of_Measure: ng/mL
      Attribute:
        Attribute_Label: Original sample concentration (ng/mL)
        Attribute_Definition: The concentration of the original sample DNA as calculated by Qubit 3.0 in nanograms per milliliter (ng/mL).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 186
            Range_Domain_Maximum: 99000
            Attribute_Units_of_Measure: ng/mL
      Attribute:
        Attribute_Label: Sample volume (μL)
        Attribute_Definition: The volume of original sample used in the assay in microliters (μL).
        Attribute_Definition_Source: USGS
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 1
            Range_Domain_Maximum: 1
            Attribute_Units_of_Measure: μL
  Distribution_Information:
    Distributor:
      Contact_Information:
        Contact_Person_Primary:
          Contact_Person: Lauren T. Toth
          Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
        Contact_Position: Research Physical Scientist
        Contact_Address:
          Address_Type: mailing and physical
          Address: 600 4Th Street South
          City: St. Petersburg
          State_or_Province: FL
          Postal_Code: 33701
          Country: US
        Contact_Voice_Telephone: 727-502-8029
        Contact_Electronic_Mail_Address: ltoth@usgs.gov
    Distribution_Liability: Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty.
    Standard_Order_Process:
      Digital_Form:
        Digital_Transfer_Information:
          Format_Name: CSV, XLSX
        Digital_Transfer_Option:
          Online_Option:
            Computer_Contact_Information:
              Network_Address:
                Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P9R8NZ2J/data/Porites_astreoides_Microsatellite_Data.zip
                Network_Resource_Name: https://coastal.er.usgs.gov/data-release/doi-P9R8NZ2J/data/Porites_astreoides_DNA_Concentration.zip
      Fees: None
  Metadata_Reference_Information:
    Metadata_Date: 20210713
    Metadata_Contact:
      Contact_Information:
        Contact_Person_Primary:
          Contact_Person: Lauren T. Toth
          Contact_Organization: U.S. Geological Survey, St. Petersburg Coastal and Marine Science Center
        Contact_Position: Research Physical Scientist
        Contact_Address:
          Address_Type: mailing and physical
          Address: 600 4Th Street South
          City: St. Petersburg
          State_or_Province: Florida
          Postal_Code: 33701
          Country: United States
        Contact_Voice_Telephone: 727-502-8029
        Contact_Electronic_Mail_Address: ltoth@usgs.gov
    Metadata_Standard_Name: Content Standard for Digital Geospatial Metadata
    Metadata_Standard_Version: FGDC-STD-001-1998
