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St. Petersburg Coastal and Marine Science Center > Joint USGS/USEPA Pathogens in Soils Geographic Information Systems Project > Research > Development of a Sampling Protocol for Bacterial Pathogens in Surface Soil

Joint USGS/USEPA Pathogens in Soils Geographic Information Systems Project

Development of a Sampling Protocol for Bacterial Pathogens in Surface Soil

The USGS and USEPA developed a protocol based on the protocol used by the NASGLP project, which describes the procedures for collecting, handling, and shipping soil samples for the detection of naturally occurring bacterial microorganisms, specifically residing within the top 0-5 cm layer of soil. This protocol has components for two different types of sampling applications: (1) typical sampling, when there is no suspicion of contamination (e.g., surveillance or background studies); and (2) in response to known or suspected contamination (e.g., the presence of animal carcasses).

Bowling, C., D. Griffin, D. Mattorano, E. Silvestri, F. Schaefer and T. Nichols, 2014, USEPA/USGS collection protocol for bacterial pathogens in surface soil. EPA/600/R-14/027. EPA600-R-14-027_EPA-USGS_SCPSOILPATHOGENS.pdf (1.7 MB PDF)

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